Total Chemical Synthesis of Proteins without HPLC Purification

Oliver Seitz describes a new method to synthesize proteins without HPLC pufirication.

The lab of Professor Oliver Seitz at the Humboldt University in Berlin (Germany) has a long-standing interest in the development of methods which facilitate the synthetic access to proteins for biological studies. Their first idea was to simplify the synthesis by means of surface-based chemistry using fully synthetic protein arrays. They were foreseeing large-scale studies on the influence of post-translational modification on protein–protein interactions. During their work towards achieving this goal they noticed an increasing interest in soluble synthetic proteins to guide the development of protein-based drugs. The method presented in this paper has many advantages over currently used methods, for example, that HPLC purification is not necessary.

Read the full article for free Total Chemical Synthesis of Proteins without HPLC Purification

Get Trial Access to the chemistry journals
Download SYNFORM or read it online