Total Chemical Synthesis of Proteins without HPLC Purification
Oliver Seitz describes a new method to synthesize proteins without HPLC pufirication.
The lab of Professor Oliver Seitz at the Humboldt University in Berlin (Germany) has a long-standing interest in the development of methods which facilitate the synthetic access to proteins for biological studies. Their first idea was to simplify the synthesis by means of surface-based chemistry using fully synthetic protein arrays. They were foreseeing large-scale studies on the influence of post-translational modification on protein–protein interactions. During their work towards achieving this goal they noticed an increasing interest in soluble synthetic proteins to guide the development of protein-based drugs. The method presented in this paper has many advantages over currently used methods, for example, that HPLC purification is not necessary.
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